EST assembling

EST assembling and clustering were performed by pipeline analysis after cleaning, repeat masking, vector trimming and organelle masking (EGassembler). Cap3 parameters were -p 95 -o 40.

A unigene set of 9 598 contigs and 26 161 singletons was obtained.

Download:

• Nicotiana tabacum ESTs available in Genbank (05/02/06) in FASTA format (13.4Mo),

• Details of Contigs obtained with these ESTs in txt format (564Ko),

• Unigene set in FASTA format (7.30Mo).

BlastX and GO annotations

BlastX with expectation value 1e^-25 and GO annotations were obtained with Blast2GO.

Download:

• Annotations in MS Excel format (1.12Mo).

Pfam domains

The unigenes were translated using ORFpredictor, and the resulting amino acid sequences where then entered as queries in the Pfam database. The default settings of Pfam 20.0 were used.

Download:

• ORFs in FASTA format (2.38Mo),

• Pfam domains in MS Excel format (488Ko).

Archives must be decompressed with WinRAR.

Additional analysis of the whole set of Nicotiana tabacum sequences released in Genbank are available on TIGR, SGN and PlantGDB, as well as the annotations relating to this last unigene set.

A transcription factor analysis is available on TOBFAC.